Quantitation of the active alpha-2-macroglobulin by trypsin protease zymography
نویسندگان
چکیده
منابع مشابه
Alpha 2 macroglobulin state in acute pancreatitis. Raised values of alpha 2 macroglobulin-protease complexes in severe and mild attacks.
Plasma values of C reactive protein, alpha 1 proteinase inhibitor, alpha 2 macroglobulin, and complexed alpha 2 macroglobulin have been determined in serial samples from 27 patients with acute pancreatitis. Complexed alpha 2 macroglobulin was measured by a novel enzyme linked immunosorbent assay with a monoclonal antibody specific for the complexed form. Patients with severe illness had lower c...
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Activation of plasma kallikrein arginine esterase activity by kaolin resulted in peak activity at 1 min of incubation and a 50% reduction in activity at 5 min in normal plasma, and 30% reduction in the plasma of patients with hereditary angioneurotic edema who lacked the C1 inactivator. The peak esterolytic activity was inhibited by soybean trypsin inhibitor whereas the 5 min activity was resis...
متن کاملInhibition of proteases by alpha 2-macroglobulin. The role of lysyl amino groups of trypsin in covalent complex formation.
The lysyl amino groups of bovine trypsin were covalently modified by acetylation, succinylation, or reductive methylation. The enzymatically active derivatives were still capable of reaction with alpha 2-macroglobulin (alpha 2M), although to a lesser extent than native enzyme. The resulting enzyme-alpha 2M complexes, however, were much more susceptible to dissociation by sodium dodecyl sulfate ...
متن کاملActive MMPs captured by alpha 2 macroglobulin as a marker of disease activity in rheumatoid arthritis.
OBJECTIVE The aim of the present study was to analyze alpha 2 Macroglobulin/MMP (alpha 2M/MMP) complex formation and to investigate whether MMP activity in alpha 2M/MMP complexes in serum can be used as a disease marker in rheumatoid arthritis (RA). METHODS High and low molecular weight (H/LMW) substrates and inhibitors and size exclusion were used to analyze alpha 2M/MMP complex formation. L...
متن کاملHigh Serum Alpha-2-Macroglobulin Level in Patients with Osteonecrosis of the Femoral Head
Background: Diagnosis of osteonecrosis of the femoral head (ONFH) is complicated due to the lack of reliable serum biomarkers. Up-regulation of alpha-2-macroglobulin (A2M) gene has been reported in glucocorticoid-induced ANFH rat model. This study aimed to investigate whether the serum level of alpha-2-macroglobulin (A2M) can be used for ONFH diagnosis. Methods: Serum protein capillary elect...
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ژورنال
عنوان ژورنال: Journal of the Serbian Chemical Society
سال: 2019
ISSN: 0352-5139,1820-7421
DOI: 10.2298/jsc190424051g